BIOLISTIC PLASTID TRANSFORMATION OF TOMATO CALLUS CELLS IN THE DEVELOPMENT OF VACCINES BASED ON THE PLANT EXPRESSION SYSTEM AGAINST THE ONCOGENE HUMAN PAPILLOMA

Original title

ПЛАСТИДНАЯ ТРАНСФОРМАЦИЯ КАЛЛУСОВ РАСТЕНИЙ ТОМАТА БИОБАЛЛИСТИЧЕСКИМ МЕТОДОМ ПРИ РАЗРАБОТКЕ ВАКЦИН ПРОТИВ ОНКОГЕННОЙ ПАПИЛЛОМЫ ЧЕЛОВЕКА НА ОСНОВЕ РАСТИТЕЛЬНОЙ ЭКСПРЕССИОННОЙ СИСТЕМЫ

Authors

A.S. Stolbikov^1,2, R.K. Salyaev¹, N.I. Rekoslavskaya^1,3

Contact information

¹Siberian Institute of Plant Physiology and Biochemistry SB RAS, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

²Irkutsk State University, Irkutsk, Russia

³Irkutsk Research Center, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

Pages

1378-1380

DOI

10.31255/978-5-94797-319-8-1378-1380

Abstract

The plastid transformation of tomato callus cells with a genetic construct containing the target gene hpv16 L1 coding the synthesis of the main antigenic papillomavirus protein HPV16 L1 was conducted. The transformation was carried out by the biolistic method. As a result, the transgenic plants expressing the antigenic protein HPV16 L1 in amount up to 5300 ng/mg total soluble protein (TSP) were obtained.