CLONAL MICROPROPAGATION OF PRUNUS TRILOBA LINDL. (FLOWERING ALMOND)

Original title

КЛОНАЛЬНОЕ МИКРОРАЗМНОЖЕНИЕ МИНДАЛЯ ТРЁХЛОПАСТНОГО (PRUNUS TRILOBA LINDL.)

Authors

A.V. Rudikovskii, K.Z. Gamburg

Contact information

Siberian institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

Pages

1353-1357

DOI

10.31255/978-5-94797-319-8-1353-1357

Abstract

The protocol for clonal micropropagation of one very attractively flowering representative of almond species – Prunus triloba Lindl was elaborated. Successful micropropagation was achieved on the medium containing half-dose MS salts, 2% sucrose, thiamin 1, pyridoxine 0.5, nicotinic acid 0.5, N6-benzylaminopurin {BAP) 0.5, 3-indolylbutyric acid (IBA) 0.1 mg·dm-3. Single shootlets were rooted with the use of the medium containing half-dose WPM salts, 2% sucrose, thiamin 1, pyridoxine 0.5, nicotinic acid 0.5 and IBA 0.5 mg·dm-3. Rooted shootlets were weaned in sterile containers with sterilized peat tablets (“gi-fi”) watered with half-dose WPM salts for two weeks and then transformed to transplants by growing them in 200 cm3 pots in the greenhouse. The transplants were grown in the field during following two years and they were ready for greening after that.