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ABOUT THE METHODS OF THE INTENSIFICATION OF THE SYNTHESIS OF ANTIGENIC PROTEINS WITH USING PLANT VIRUS REGULATORY ELEMENTS AT THE DEVELOPMENT OF INNOVATIVE VACCINES ON THE BASE OF PLANT EXPRESSION SYSTEMS
Original title
О СПОСОБАХ ИНТЕНСИФИКАЦИИ СИНТЕЗА АНТИГЕННЫХ БЕЛКОВ С ИСПОЛЬЗОВАНИЕМ ВИРУСНЫХ РЕГУЛЯТОРНЫХ ЭЛЕМЕНТОВ ПРИ РАЗРАБОТКЕ ИННОВАЦИОННЫХ ВАКЦИН НА БАЗЕ РАСТИТЕЛЬНЫХ ЭКСПРЕССИОННЫХ СИСТЕМ
Authors
N.I. Rekoslavskaya1,2, R.K. Salyaev1, А.S. Stolbikov1
Contact information
1Siberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2Irkutsk Scientific Center of Russian Academy of Sciences, Irkutsk, Russia
Pages
1348-1352
DOI
10.31255/978-5-94797-319-8-1348-1352
Abstract
To enhance the synthesis of antigenic protein HPV16 L1 of high-risk oncogenic type papillomavirus 16, the plant expression synthesis was used on the base of transgenic tomato fruit. To create stable transformation, the genetic construct pBINHPV16 L1 Ω 5'UTR TMV was used that provided the yield of HPV16 L1 up to 2330 ng per 1 mg of total soluble protein (TSP). For transient expression, the genetic construct pBIN p35S RdRP CMV (RNA 2 a,b) HPV16 L1 was developed that included the antisilencing protein 2b. In this case the yield of antigenic protein HPV16 L1 was rised to 200000 ng per mg TSP.