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DEVELOPING OF METHOD OF FLUORESCENT LABELING OF DNA FRAGMENTS WITH DIFFERENT LENGTHS AND ANALYSIS OF LABELED SUBSTRATES IMPORT IN THE MITOCHONDRIA OF PLANTS
Original title
ПОСТАНОВКА МЕТОДА ФЛУОРЕСЦЕНТНОГО МЕЧЕНИЯ ФРАГМЕНТОВ ДНК РАЗНОЙ ДЛИНЫ И АНАЛИЗА ИМПОРТА МЕЧЕНЫХ СУБСТРАТОВ В МИТОХОНДРИИ РАСТЕНИЙ
Authors
A.V. Panfilov1, Yu.M. Konstantinov1,2, M.V. Koulintchenko2
Contact information
1Irkutsk State University, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2Siberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia, mk100171@yahoo.com
Pages
1339-1343
DOI
10.31255/978-5-94797-319-8-1339-1343
Abstract
We applied two approaches to obtain the fluorescent labeled DNA as substrates for mitochondria import: (1) introduction of the fluorescent dye in DNA molecules using PCR amplification with primers containing Cy3: (2) incorporation in DNA of modified mononucleotide aminoallyl-dUTP during PCR, followed by postsynthetic labeling of the AA-dUTP by activated Nhydroxysuccinimide ester (Cy3-SE). We tested the efficiency of import into mitochondria isolated from A. thaliana of DNA substrates labeled thus in PCR.