full text (pdf)
ENHANCERS OF PROKARYOTIC TRANSLATION UNDER THE TEMPERATURE STRESS
Original title
ЭНХАНСЕРЫ ПРОКАРИОТИЧЕСКОЙ ТРАНСЛЯЦИИ В УСЛОВИЯХ ТЕМПЕРАТУРНОГО СТРЕССА
Authors
A.S. Nizkorodova1, R.U. Musabaev1, N.Zh. Karimov2, B.K. Iskakov1
Contact information
1State Research Institute of Molecular Biology and Biochemistry named after M.A. Aytkozhin, Almaty, Kazakhstan, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2State Research Institute of Plants’ Biology and Biotechnology, Almaty, Kazakhstan, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
Pages
1316-1320
DOI
10.31255/978-5-94797-319-8-1316-1320
Abstract
The novel translation stimulator TPS (Translation-initiation Promoting Site), presented in the 5'-untranslated sequences of prokaryotic mRNAs with a high expression level, was studed. TPS is complimentary to the 5'-GGAUCA-3' site at the 3'-end of 16S rRNA. When TPS was combined with Shine-Dalgarno (SD), a significant increase in expression level was shown in comparison with SD alone in E. coli cells under stress conditions – 5.5 times greater at 25 °C, and almost 2-fold greater at 44 °C.