USE TRANSIENT EXPRESSION FOR STUDY OF THE LOCALIZATION AND FUNCTIONAL ROLE OF PROTEINS IN A PLANT CELL, ON THE EXAMPLE OF ACYL-LIPID Δ9-DESATURASE

Original title

ИССЛЕДОВАНИЕ ЛОКАЛИЗАЦИИ И ФУНКЦИОНАЛЬНОЙ РОЛИ БЕЛКОВ В РАСТИТЕЛЬНОЙ КЛЕТКЕ С ПОМОЩЬЮ ТРАНЗИЕНТНОЙ ЭКСПРЕССИИ, НА ПРИМЕРЕ АЦИЛ-ЛИПИДНОЙ Δ9-ДЕСАТУРАЗЫ

Authors

M.A. Berestovoy, O.S. Pavlenko, A.A. Tyurin, R.A. Sidorov, I.V. Goldenkov-Pavlova

Contact information

K.A. Timiryazev Institute of Plant Physiology RAS, Moscow, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

Pages

1183-1186

DOI

10.31255/978-5-94797-319-8-1183-1186

Abstract

We studied the localization and functional role of the target protein in the plant cell using unified vectors for transient expression with the gene sequence of Δ9 desaturase and leader sequences for localization the protein product of the gene in the cytoplasm, chloroplasts, and ER. The study includes fine visualization of the target protein in cell compartments and an estimation of the changes in composition and mass fraction of fatty acids, depending on its localization.