IN SILICO IDENTIFICATION OF SEQUENCES ENCODING CLC FAMILY PROTEINS IN THE DE NOVO ASSEMBLED TRANSCRIPTOMES OF MARINE ALGA DUNALIELLA TERTIOLECTA

Original title

ИДЕНТИФИКАЦИЯ IN SILICO ПОСЛЕДОВАТЕЛЬНОСТЕЙ, КОДИРУЮЩИХ БЕЛКИ СЕМЕЙСТВА CLC, В СОБРАННЫХ DE NOVO ТРАНСКРИПТОМАХ МОРСКОЙ МИКРОВОДОРОСЛИ DUNALIELLA TERTIOLECTA

Authors

L.G. Popova¹, A.V. Shuvalov¹, A.A. Yurchenko², D.E. Khramov^1,3, D.A. Matalin¹, L.A. Khalilova¹, Y.V. Orlova¹, Y.V. Balnokin^1,3

Contact information

¹K.A. Timiryazev Institute of Plant Physiology RAS, Moscow, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

²Federal State Budgetary Educational Institution of Higher Education "Saint-Petersburg State University", Theodosius Dobzhansky Center for Genome Bioinformatics, SaintPetersburg, Russia

³Federal State Budget Educational Institution of Higher Education M.V. Lomonosov Moscow State University, Moscow, Russia

Pages

978-982

DOI

10.31255/978-5-94797-319-8-978-982

Abstract

For microalgae Dunaliella tertiolecta de novo transcriptome assemblies were accomplished using the short-read RNA-seq data deposited in the Sequence Read Archive database (NCBI). In the assembled transcriptomes by in silico analysis we found sequences encoding the Cl^-- transporting proteins of the CLC family: DtCLC1 (857 a.a.), DtСLС2 (809 a.a.), DtCLC3 (768 a.a.). These proteins are similar to the CLC proteins of higher plants and animals (primarily fish and reptiles) that fulfil Cl^-/H⁺ exchange.