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CLONING AND FUNCTIONAL CHARACTERISTIC OF THE SaCLCa1 AND SACLCc1 GENES OF CHLORIDE CHANNEL FAMILY (CLC) FROM HALOPHYTE SUAEDA ALTISSIMA (L.) Pall.
Original title
КЛОНИРОВАНИЕ И ФУНКЦИОНАЛЬНАЯ ХАРАКТЕРИСТИКА ГЕНОВ SaCLСa1 И SaCLCc1 СЕМЕЙСТВА ХЛОРИДНЫХ КАНАЛОВ (CLC) ИЗ ГАЛОФИТА SUAEDA ALTISSIMA (L.) Pall
Authors
O.I. Nedelyaeva1, А.В. Shuvalov1, Yu.V. Orlova1, L.A. Khalilova1, D.V. Belyev1, N.A. Myasoedov1, O.V. Mayorova1, A.A. Yurchenko2, L.G. Popova1, Yu.V. Balnokin1,3, I.V. Karpichev1
Contact information
1K.A. Timiryazev Institute of Plant Physiology RAS, Moscow, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2Federal State Budgetary Educational Institution of Higher Education "Saint-Petersburg State University", Theodosius Dobzhansky Center for Genome Bioinformatics, SaintPetersburg, Russia
3Federal State Budget Educational Institution of Higher Education M.V. Lomonosov Moscow State University, Moscow, Russia
Pages
952-956
DOI
10.31255/978-5-94797-319-8-952-956
Abstract
SaCLCa1 and SaCLCc1, the genes of the CLC (chloride channel) family were cloned from halophyte Suaeda altissima (L.) Pall.. To study functions, SaCLCa1 and SaCLCc1 were expressed in the mutant Saccharomyces cerevisiae Δgef1. Expression of SaCLCc1 in Δgef1 led to restoration of mutant growth on selective media. Expression of SaCLCa1 restored the growth of Δgef1 on one of the media only when proline was replaced with serine in the selective filter at position 188. SaCLCa1 and SaCLCc1 were expressed in all organs of S. altissima. Deficiency of NO3- in the medium stimulated the expression of SaCLCa1, especially noticeable in the roots. It is assumed that SaCLCa1 transports NO3-, whereas SaCLCc1 - Cl-.