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S-RNase-BASED SELF-INCOMPATIBILITY AND PROGRAMMED CELL DEATH
Original title
САМОНЕСОВМЕСТИМОСТЬ РНКазного ТИПА И ПРОГРАММИРОВАННАЯ КЛЕТОЧНАЯ СМЕРТЬ (PCD)
Authors
L.V. Kovaleva1, E.V. Zakharova2,3, G.V.Timofeeva1
Contact information
1K.A. Timiryazev Institute of Plant Physiology RAS, Moscow, Russia,
2Federal State Budgetary Educational Institution of Higher Education “Russian Timiryazev State Agrarian University”, Moscow, Russia,
3All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia,
Pages
931-935
DOI
10.31255/978-5-94797-319-8-931-935
Abstract
PCD markers were revealed in the pollen-pistil system of petunia characterized by SRNase-based self-incompatibility. Three methods were used: trypan blue, DNA-electrophoresis and TUNEL method. Additional staining with DAPI showed that TUNEL-positive signal originates from nuclear DNA. The results reliably prove that PCD is a determinant of S-RNase-based selfincompatibility in petunia.