STAY-GREEN PHENOTYPE IN THE ARABIDOPSIS THALIANA GLUTAMATE DEHYDROGENASE MUTANT GDH1GDH2

Original title

ФЕНОТИП "STAY-GREEN" У МУТАНТА ARABIDOPSIS THALIANA ПО ГЕНАМ ГЛУТАМАТДЕГИДРОГЕНАЗЫ GDH1GDH2

Authors

E.Yu. Garnik¹, D.V. Vilyanen², A.A. Vlasova², V.I. Belkov¹, V.I. Tarasenko¹, Yu.M. Konstantinov¹

Contact information

¹Siberian Institute of Plant Physiology and Biochemistry of Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

²Irkutsk State University, Irkutsk, Russia

Pages

903-907

DOI

10.31255/978-5-94797-319-8-903-907

Abstract

Dark-induced senescence of plants is a process of leaf yellowing and dying-off in conditions of the long-term dark. We detected that the double knock-out Arabidopsis mutant gdh1gdh2 retains a mainly green color of its leaves when incubated in the dark during four days and more, whereas wild type Arabidopsis plants turn yellow in the same conditions. Chlorophyll content decreased during 7 days in the dark to 40-50% of the initial level in the wild type Arabidopsis line Col-0 but only to 70-80% of the initial level in the gdh1gdh2 plants. Chlorophyll a/b rate decreased in the gdh1gdh2 plants as a consequence of the slower chlorophyll b degradation. Gene expression analysis in green, yellowing and yellow Col-0 leaves and in typical (green) gdh1gdh2 leaves after 5-6 days in the dark demonstrated that the slow chlorophyll content fall in the mutant plants is a result of the low expression of genes NYC1, PAO, PPH, SGR1 and SGR2 responsible for chlorophyll degradation. So, the genetic program of chlorophyll destruction during a dark-induced senescence is disrupted in the gdh1gdh2 mutant plants.