DEVELOPMENT OF METHODS FOR CRYOCONSERVATION OF VEGETATIVE SHOOTS (CUTTINGS) EMPLOYING VARIETIES OF RED CURRANT (R. RUBRUM L.) FROM THE GENE POOL PRESERVED AT THE POLAR EXPERIMENT STATION OF THE VAVILOV INSTITUTE

Original title

DEVELOPMENT OF METHODS FOR CRYOCONSERVATION OF VEGETATIVE SHOOTS (CUTTINGS) EMPLOYING VARIETIES OF RED CURRANT (R. RUBRUM L.) FROM THE GENE POOL PRESERVED AT THE POLAR EXPERIMENT STATION OF THE VAVILOV INSTITUTE

Authors

V.G. Verzhuk, A.V. Pavlov, L.V. Sukhareva, I.I. Gracheva, T.D. Kuvaeva

Contact information

The N.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

Pages

858-860

DOI

10.31255/978-5-94797-319-8-858-860

Abstract

The work on cryoconservation of vegetative shoots and buds of red currant was based on the technique developed by P. Forsline for dormant apple-tree buds, which we modified to make it applicable to our plant. Cuttings were selected from the collection of small fruit plants held by the Polar Experiment Station of VIR. They were cut into sections 7–8 cm long with 2–3 buds, and dried in an incubator at –4–5°C down to 28–32% moisture content. After that they underwent two-step freezing in Sanyo Medical Freezer MDF-U442(T), and were placed for long-term storage into liquid nitrogen vapor. Assessment of the cuttings’ viability prior to their placement into nitrogen (initial), and after their storage and planting in the field showed their differences and variety-specific dependence. The minimum initial value for this parameter was 56.6±5.0% demonstrated by var. Natali (No. K-202), while the maximum of 90.0±3.0% was shown by var. Laplandia (No. K-315). The same correlation between these varieties was recorded after their cryopreservation and taking root in the field: 46.6±5.0% for var. Natali, and 76.6±4.2% for var. Laplandia. The remaining varieties demonstrated intermediate values, but had a rather good percentage of rooting after cryostorage.