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OBTAINING AND BIOCHEMICAL EVALUATION OF SUGAR BEET REGENERANTS WITH RESISTANCE TO EDAPHIC FACTORS
Original title
ПОЛУЧЕНИЕ И БИОХИМИЧЕСКАЯ ОЦЕНКА РЕГЕНЕРАНТОВ САХАРНОЙ СВЁКЛЫ С УСТОЙЧИВОСТЬЮ К ЭДАФИЧЕСКИМ ФАКТОРАМ
Authors
O.A. Zemlyanuhina1, N.N. Cherkasova2, T.P. Zhuzhzhalova2, V.N. Kalaev1
Contact information
1Voronezh State University, Voronezh, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2The A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar (VNIISS), vil. VNIISS, Voronezh region, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
Pages
354-357
DOI
10.31255/978-5-94797-319-8-354-357
Abstract
Based on stress selection, a method to obtain resistant sugar beet regenerants of different genotypes under in vitro conditions has been developed. Stress factors are low moisture content and high acidity level (use of chloraluminite) of Gamborg’s nutrient medium. First stage includes in vitro cultivation of mature seed embryos with addition of the selective agent sorbite in concentrations of 0.45 M and with рН 3.5 to nutrient medium. Original and secondary selections of regenerants with resistance to edaphic factors are carried out on this medium. Biochemical evaluation of the selected resistant material is conducted.