ASSOCIATION OF HIGH-LIGHT-INDUCIBLE SMALL STRESS PROTEINS WITH COMPLEXES OF THYLAKOID MEMBRANES

Original title

АССОЦИАЦИЯ СТРЕССОВЫХ НИЗКОМОЛЕКУЛЯРНЫХ СВЕТОИНДУЦИРУЕМЫХ БЕЛКОВ С КОМПЛЕКСАМИ ТИЛАКОИДНЫХ МЕМБРАН

Authors

L.S. Sharapova, N.P.Yurina

Contact information

Bach Institute of Biochemistry, Research Center of Biotechnology RAS, Moscow, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

Pages

153-154

DOI

10.31255/978-5-94797-318-1-153-154

Abstract

Although light is essential for photosynthesis, an excess of light energy can damage the photosynthetic apparatus in a process of photooxidative damages. High-light-inducible proteins (Hlip) are important in protection of the photosynthetic apparatus of cyanobacteria from light stress. The association of high-light-inducible (HliA/HliB) small stress proteins with photosystem 1 (PS1) complexes of the cyanobacteria was studied to understand their function. Two cyanobacteria Synechocystis sp. РСС 6803 and Arthrospira platensis were studied. Chlorophyll-protein complexes were fractionated by two-dimension polyacrylamide gel electrophoresis (2D PAGE). Proteins identification was performed by MALDI-TOF mass spectrometry and by Western-blot analysis. The trimers and monomers of photosystem 1 (PS1), dimers and monomers of the photosystem 2 (PS2), the cytochrome complex, ATP-ase, the NAD(P)H-quinone-oxidoreductase and zone of the free protein were identified by 2D PAGE. HliA/HliB proteins were associated with chlorophyll-protein complexes and were shown in the free protein zone by immunoblotting. Hli proteins associated with trimers PS1, complexes of PS2 and monomers PS1. Hli proteins were also found in the free proteins zone. The association of HliA/HliB proteins not only with PSI trimers, but also with PS1 monomers and PS2 complexes was shown. This fact suggests a universal role of these proteins in the protection of the photosynthetic apparatus from light stress.