SUBCELLULAR LOCALIZATION OF CYCLIC NUCLEOTIDE-GATED ION CHANNELS (CNGCS) IN THE POTATO ROOT CELLS IN THE BIOTIC STRESS

Original title

СУБКЛЕТОЧНАЯ ЛОКАЛИЗАЦИЯ РЕГУЛИРУЕМЫХ ЦИКЛИЧЕСКИМИ НУКЛЕОТИДАМИ ИОННЫХ КАНАЛОВ (CNGC) В КЛЕТКАХ КОРНЕЙ КАРТОФЕЛЯ ПРИ БИОТИЧЕСКОМ СТРЕССЕ

Authors

A.S. Romanenko, L.A. Lomovatskaya, N.V. Filinova

Contact information

Siberian Institute of Plant Physiology and Biochemistry SB RAS

Pages

106-108

DOI

10.31255/978-5-94797-318-1-106-108

Abstract

Using the electron immunocytochemistry method, localization of cyclic nucleotide-gated Ca2+-permeable ion channels (presumably, CNGC18 channel) is shown in plasma membrane, tonoplast, and membranes of mitochondria and endoplasmic reticulum of the potato plant root cells. Treatment of the pathogen-resistant potato cultivar with exopolysaccharides from the bacterial pathogen causing potato ring rot, led to a decrease in the number of gold particles, reflecting the localization of the channels, in the plasma membrane and to an increase of their number in the outer mitochondrial membranes. In contrast, in cells of the pathogen-susceptible cultivar, exopolysaccharides induced an increase in the number of gold particles in plasma membrane and mitochondrial cristae. In the tonoplasts of cells of both cultivars the gold particles were practically absent. Possible roles of the cyclic nucleotide-gated channels in the regulation of physiological and biochemical processes in plant cells involving Ca2+ and adenylyl cyclase signaling system are discussed.