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REDOX-ACTIVE LOW MOLECULAR WEIGHT COMPOUNDS OF RED BEET-ROOT VACUOLES, PLASTIDS AND MITOCHONDRIA
Original title
РЕДОКС-АКТИВНЫЕ НИЗКОМОЛЕКУЛЯРНЫЕ СОЕДИНЕНИЯ ВАКУОЛЕЙ, ПЛАСТИД И МИТОХОНДРИЙ КЛЕТОК КОРНЕПЛОДОВ СТОЛОВОЙ СВЕКЛЫ
Authors
E. V. Pradedova1, O. D. Nimaeva1, A. B. Karpova2, R. K. Salyaev1
Contact information
1Siberian Institute of Plant Physiology and Biochemistry SB RAS, Irkutsk, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2Faculty of Biology and Soil Science, Irkutsk State University, Irkutsk.
Pages
97-99
DOI
10.31255/978-5-94797-318-1-97-99
Abstract
Redox state of cell compartments is caused by the redox status of glutathione (GSH/GSSG), ascorbic acid (AA/DHA), and to a certain extent by the redox status of dinucleotides (NAD(P)+/NAD(P)H). A study was undertaken to determinate the redox-status of vacuoles, plastids and mitochondria of beetroot cells (Beta vulgaris L.) at the stage of physiological dormancy of root crops. The contents of the reduced and oxidized glutathione, ascorbic acid and NAD(H) was determined in isolated organelles. And, it turned out that in vacuoles the pool of ascorbic acid and glutathione was more oxidized than in plastids and mitochondria. The more reduced state of glutathione and ascorbic acid was characteristic of mitochondria. All these reasons pointed in the prevalence of oxidative processes in vacuoles, and the pre-dominance of the reductive processes in mitochondria. These results have made it possible fi-nally to conclude that most significant component of redox buffer from vacuoles is ascorbic acid, and most significant component of redox buffer from mitochondria and plastids is gluta-thione. In addition, NAD has a significant contribution to the formation of redox conditions in plastids. In general, it was confirmed that the pool of major redox compounds is more oxi-dized in vacuoles than in plastids and mitochondria.