COULD STEM CELLS ACT AS ORGANELLES DONORS: CELL-TO-CELL MITOCHONDRIA TRANSPORT

Original title

МОГУТ ЛИ СТВОЛОВЫЕ КЛЕТКИ БЫТЬ ДОНОРАМИ ОРГАНЕЛЛ: МЕЖКЛЕТОЧНЫЙ ТРАНСПОРТ МИТОХОНДРИЙ

Authors

Plotnikov E.Y.^1,2, Babenko V.A.^1,2, Silachev D.N.^1,2, Pevzner I.B.^1,2, Zorova L.D.^1,2, Sukhikh G.T.², Zorov D.B.^1,2

Contact information

¹ A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

² V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow, 117997, Russia.

Pages

92-93

DOI

10.31255/978-5-94797-318-1-92-93

Abstract

Since the possibility of intercellular transport of mitochondria was discovered in 2006 an active studies of such transfer were performed, and the mechanisms of organelle transport between different cell types were widely described. We studied the mitochondria transfer between mesenchymal multipotent stromal cells (MMSCs) and various types of differentiated somatic cells. The transport of mitochondria was studied in the model of MMSC co-cultivation with differentiated cells. We observed the transfer of mitochondria from MMSC to neurons, renal epithelium and cardiomyocytes. The transfer was confirmed by immunofluorescence with species-specific antibodies against human cytochrome oxidase and with genetically-encoded mitochondria-targeted fluorescent proteins. Transfer of low-molecular components of the cytoplasm between the cells was also observed. The direction of cytoplasm transport was different in various cellular systems; however, in all systems the direction of mitochondrial transfer was the same - from MMSC to the recipient cell. As a result of mitochondria transfer the restoration of bioenergetic functions in cells was revealed. Thus, we have shown that MMSC can be the donors of functionally active mitochondria, transferring them to various somatic cells. This transfer of mitochondria probably influences the restoration of mitochondrial function in recipient cells that were damaged under stress conditions.