THE EXPRESSING OF THE DESA Δ12-ACYL-LIPID DESATURASE GENE OF SYNECHOCYSTIS CHANGES THE STRUCTURE OF POTATO CHLOROPLASTS UNDER THE HARDENING TO LOW TEMPERATURE

Original title

ВВЕДЕНИЕ ГЕНА DESA Δ12-АЦИЛ-ЛИПИДНОЙ ДЕСАТУРАЗЫ SYNECHOCYSTIS ВЛИЯЕТ НА СТРУКТУРУ ХЛОРОПЛАСТОВ ПРИ ЗАКАЛИВАНИИ КАРТОФЕЛЯ К ГИПОТЕРМИИ

Authors

N. V. Naraikina, T. I. Trunova

Contact information

K. A. Timiryazev Institute of Plant Physiology RAS, Russia, Moscow

Pages

66-68

DOI

10.31255/978-5-94797-318-1-66-68

Abstract

The aim of this study was to investigate changes of the ultrastructural organization of potato chloroplasts and the level of resistance to hypothermia induced by low temperature hardening and the role of the Δ12-acyl-lipid desaturase of cyanobacteria in this processes. Plants were hardened at 5°C, for 6 days, with dinnual 16-hour light. Using the methods of electron microscopy and morphometry, it was shown decreasing square cut of chloroplast and total area of starch grains by almost 40% after hardening of the control plants. The total area of plastoglobuli and the total number of tylakoids, designed for one chloroplast, were increased by 80 and 35%. Transformants before hardening differed from the control plants almost twice as many thylakoids membranes. And after hardening significant changes in the membrane system of chloroplasts have not been identified. But the total number of thylakoids in them was higher in comparison with the control due to changes as a result of transformation. Thus, transformers already before hardening possessed some signs of the hardened plants, for example, differed in a large number of tilakoid membranes. These results confirm the role of Δ12-desaturase in maintaining the functional activity of membranes as the temperature decreases.