AGROBACTERIUM-MEDIATED TRANSFORMATION OF TRANSPLASTOMIC TOBACCO PLANTS

Original title

АГРОБАКТЕРИАЛЬНАЯ ТРАНСФОРМАЦИЯ ТРАНСПЛАСТОМНЫХ РАСТЕНИЙ ТАБАКА

Authors

Kuluev B.R., Knyazev A.V., Vershinina Z.R., Baymiev Al.K., Chemeris A.V.

Contact information

Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

Pages

61-62

DOI

10.31255/978-5-94797-318-1-61-62

Abstract

The first transgenic plants were created in 1977, around the same time the first hairy roots were generated. The first transplastomic plants were generated in 1990. After 25 years after that, we decided to combine technologies for obtaining transplastomic plants and hairy roots. The aim of our work was to generate transplastomic tobacco plants, as well as genetic transformation of these plants by Agrobacterium rhizogenes. For the generation of transplastomic tobacco plants (Nicotiana tabacum), the pKMS8 vector was used with the spectinomycin resistance gene (aadA) without target genes. Three different lines of spectinomycin and streptomycin-resistant tobacco plants were generated in the course of the work, transplastomic transformation of which was confirmed by PCR, analysis of the test for resistance of shoots to spectinomycin, and analysis of the transmission of antibiotic resistance through pollen. Next, leaf explants of transplastomic tobacco plants were used for agrobacterium-mediated transformation using strain 15834 of A. rhizogenes. Hairy roots of tobacco, capable of growth on a nutrient medium with spectinomycin, were generated. The PCR analysis of the hairy roots showed in them the presence both rol-genes and aadA gene. It is suggested that transplastomic hairy roots can become a promising plant system for the production of recombinant proteins.