STUDYING OF DNA IMPORT FACTORS IN PLANT MITOCHONDRIA

Original title

ИССЛЕДОВАНИЕ ФАКТОРОВ ИМПОРТА ДНК В МИТОХОНДРИИ РАСТЕНИЙ

Authors

Yu.M. Konstantinov^1,2, M.V. Koulintchenko¹, E.S. Klimenko¹, N.A. Bolotova¹, V.I. Tarasenko¹, V.N. Shmakov¹

Contact information

¹Siberian Institute of Plant Physiology and Biochemistry SB RAS, Irkutsk, Russia, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

²Irkutsk State University, Irkutsk, Russia

Pages

55-57

DOI

10.31255/978-5-94797-318-1-55-57

Abstract

The aim of the study was identification of protein factors involving in the natural mechanism of DNA transport through outer and inner membranes of mitochondria. The investigation of voltage-dependent anion channel (VDAC) involvement in the DNA import mechanism using Arabidopsis mutant plants with inactivated At3g01280 and At5g15090 genes, coding VDAC1 and VDAC3 isoforms, showed that the mitochondria lacking either of these two porin isoforms transport DNA more efficiently compared to the wild-type mitochondria. Increased activity of the DNA import into mitochondria of these mutants was not specific in terms of the length of transported DNA: the import process of three used DNA substrates, of short (269 bp) and middle (852 and 2732 bp) length, was activated. It was also shown that cell microsomal fraction stimulates the DNA transport into mitochondria. Activation of the DNA import into mitochondria of the dicotyledonous plants representative (Solanum tuberosum) in the presence of microsomes isolated from dicotyledonous plants was significantly higher in comparison with the effect of microsomes isolated from a monocots plant representative (Zea mays). These results indicate the importance of interactions between mitochondria and endoplasmic reticulum for the DNA import into mitochondria in vivo. Using Arabidopsis protoplasts transfection, it was found that the DNA of various lengths is actively imported from the cytoplasm to the mitochondria in vivo.