STUDYING OF ROLE OF THE INTERACTION BETWEEN MITOCHONDRIA MEMBRANES AND ENDOPLASMATIC RETICULUM IN DNA IMPORT

Original title

ИЗУЧЕНИЕ РОЛИ ВЗАИМОДЕЙСТВИЯ МЕМБРАН МИТОХОНДРИЙ И ЭНДОПЛАЗМАТИЧЕСКОГО РЕТИКУЛУМА В ИМПОРТЕ ДНК

Authors

E.S. Klimenko, V.N. Shmakov, T.A. Bolotova, I.Yu. Subota, V.I. Tarasenko, M.V. Koulintchenko, Yu.M. Konstantinov

Contact information

Siberian Institute of Plant Physiology and Biochemistry SB RAS, Irkutsk, Russia, e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

Pages

49-51

DOI

10.31255/978-5-94797-318-1-49-51

Abstract

We have previously shown that isolated plant mitochondria have the ability to import DNA molecules (Koulintchenko et al, 2003). Nevertheless, it cannot be ruled out whether the structural and functional complexes in the membrane contact zones involved in metabolism of various metabolites between ER and mitochondria might also have an impact on the DNA transport into mitochondria. For this purpose the in organello system of DNA import was complemented by addition of a cell microsomal fraction (fragmented ER membranes). It was shown that microsomal fraction stimulates the DNA transport into mitochondria. Obviously, it is the proteins of the mitochondrial outer membrane that play a key role in formation of structural and functional complexes between the membrane structures of mitochondria and ER. Activation of the DNA import into Solanum tuberosum mitochondria, a representative of dicotyledonous plants in the presence of microsomes isolated from dicotyledonous plants (S. tuberosum and A. thaliana) was significantly higher in comparison with the effect of microsomes isolated from a representative of monocots plant (Zea mays). We assume that, in addition to mitochondrial proteins, ER proteins can also participate in formation of certain channels / pores that facilitate the import of DNA into mitochondria in vivo. These results indicate the importance of interactions between mitochondria and endoplasmic reticulum for the DNA import into mitochondria in vivo.