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STUDY OF CHLOROPHYLL METABOLISM IN A DOUBLE ARABIDOPSIS THALIANA MUTANTGDH1GDH2 DURING A LONG-TERM EXPOSITION OF PLANTS IN THE DARK
Original title
ИЗУЧЕНИЕ ОСОБЕННОСТЕЙ МЕТАБОЛИЗМА ХЛОРОФИЛЛОВ У ДВОЙНОГО НОКАУТ-МУТАНТА ARABIDOPSIS THALIANA ПО ГЕНАМ ГЛУТАМАТДЕГИДРОГЕНАЗЫ ПРИ ДЛИТЕЛЬНОМ ВЫДЕРЖИВАНИИ РАСТЕНИЙ В ТЕМНОТЕ
Authors
D.V. Vilyanen1, E. Yu. Garnik2, V. I. Tarasenko2, Yu. M. Konstantinov2
Contact information
1Irkutsk State University, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2 Siberian Institute of Plant Physiology and Biochemistry SB RAS, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
Pages
22-24
DOI
10.31255/978-5-94797-318-1-22-24
Abstract
Chlorophyll content and composition is a result of two simultaneous processes, chlorophyll synthesis and degradation. In higher plants this processes are depend on both the developmental stage and environmental conditions. A long-term (more than 4 days) exposition of Arabidopsis plants in the dark leads commonly to leaf yellowing (dark-induced senescence). But we have found that the Arabidopsis double knock-out mutant gdh1gdh2 leaves are yellowed much more slowly in such conditions. Chlorophyll content decreased during 7 days in the dark to 40-50% in the wild type Arabidopsis line Col-0 and to 70-80% in gdh1gdh2 plants. Chlorophyll a/b rate in gdh1gdh2 plants demonstrated a tendency to decrease as a consequence of the slower chlorophyll b degradation. Expression of genes responsible for the early steps of chlorophyll synthesis (GSA1, HEMA1, GRS) has no significant differences between Col-0 and gdh1gdh2 during 7 days in the dark. Expression of genes NYC1, PAO and SGR2, responsible for chlorophyll degradation, was lower in gdh1gdh2 plants after 4 and 7 days in the dark. Hence the slow chlorophyll content fall is a result of disturbed chlorophyll degradation rather than enhanced synthesis in the dark.